Invivotek

Full List of In Vivo Assays for Pre-Clinical Efficacy Evaluation


Invivotek’s preclinical compound evaluation program offers direct disease-related physiological and behavioral end-points for efficacy assessment of test therapeutics. We offer tests in rats and mice to identify potential therapeutics to treat autoimmune, inflammatory, metabolic, cardiovascular and neurodegenerative disorders. When necessary we can help with optimization of experimental design and selection of specific test to achieve your goals. Invivotek has the capabilities to perform pharmacokinetic and limited toxicological evaluations to select optimal doses for efficacy testing.

Metabolism, Obesity and Diabetes

All of the assays can be performed on regular and/or high fat diets

Body Weight and Body Composition
Longitudinal Body Weight Gain
Body Composition by DEXA:

  • Fat mass/ % fat/ lean mass/ % lean/ total tissue mass/ tissue area
  • Bone Mineral Density and Content/ bone area

Assessment of Regional Adipose Depots
Aipocyte tissue macrophage measurement by FACS
Energy Homeostasis
Food Intake

Indirect Calorimetry and Locomotor Activity:

  • O2 Consumption/ CO2 Production/ Respiratory Exchange Ratio (RER)
  • Total Horizontal / Ambulatory/ Non-ambulatory/ Vertical Activity
  • Licking Frequency

Glucose Homeostasis
Glucose and Insulin Levels in the Oral Glucose Tolerance Test
Gastric Emptying
Insulin Tolerance Test
Tissue Glycogen Levels
Pancreatic Insulin Content
Glucagon Levels
Serum Adiponectin Levels

Specialized Blood Chemistries
Serum Corticosterone Levels
Serum Leptin Levels
Serum Ketone Levels
Serum Triglyceride Levels
Serum Free Fatty Acids
Serum Cholesterol Levels
Cholesterol Distribution: VLDL, LDL, HDL

Cardiovascular

Hypertension
Non-invasive Blood Pressure & Heart Rate
Exercise capacity
High Salt or High Fat Dietary Challenge
L-NMMA Induced Hypertension

Thrombosis
Activated Partial Thromboplastin Time (aPTT)
Collagen-induced Platelet Aggregation
Prothrombin Time (PT)
Tail Bleeding Time

Immunology & Inflammation

Animal Disease Models
Antigen-induced Arthritis for Rheumatoid Arthritis
Collagen-Induced Arthritis for Rheumatoid Arthritis
KRN-serum Induced Arthritis for Rheumatoid Arthritis
Monoclonal Antibody Induced Arthritis for Rheumatoid Arthritis
Spontaneous Arthritis in huTNF alpha transgenic mice
Lethal Endotoxemia (Septic Shock)
Experimental Autoimmune Encephalitis for Multiple Sclerosis
Allergen-induced Pulmonary Inflammation for Asthma
Lipopolysaccharides (LPS)-induced Airway Inflammation
Acute lung injury model (LPS-induced)
DSS-induced Colitis for Ulcerative Colitis
TNBS-induced Colitis for Crohn’s Disease
Allergic Dermatitis (DNFB or Oxazolone induced)

Other in vivo Bioassays
Non-lethal Endotoxemia with LPS
Antigen-dependent In Vivo T-cell Proliferation by BrdU uptake
OVA-stimulated In Vivo Antibody Production (IgG1, IgG2a, IgG2b)
KLH-stimulated In Vivo Antibody Production (IgM, IgG)
Delayed Type Hypersensitivity by mBSA or KLH with adjuvant
Delayed Type Hypersensitivity by SRBC
Monocyte or Neutrophil Infiltration induced by Thioglycollate or Zymosan or Curdlan or Tripsin
Cutaneous Wound Healing

Ex vivo Bioassays
Serum and Tissue Cytokine Profiling in animal disease models
FACS Analysis of Lymphoid Organs and Tissue Infiltrates in Naïve and Disease Induced Animals
Hematology Five Parts Differential Cell Count
In vitro B cell proliferation Induced by LPS
In vitro T cell Proliferative Responses Induced by Anti-CD3/CD28
Cytokine Production by T cells after stimulation with anti-CD3/CD28
Cytokine Production by Monocytes after stimulation with LPS
Cytotoxic T-cell Activity (CTL)
LAK- Lymphokine-Activated Killer Cell Cytotoxicity
NK- Natural Killer Cell Activity
Th17 Polarizing Conditions from CD4+ T cells (ELISA for Il-17 and IL-22)
Treg Polarization from CD4+ T cells (ELISA for IL-10)
Th1 Differentiation from CD4+ T cells (ELISA for INF-gamma)
Th2 Differentiation from CD4+ T cells (ELISA for IL-4)

Oncology

Orthotopic Xenograft and Syngeneic Tumor Models with Spontaneous Metastases
Experimental Bone Metastases Models by intra-cardiac injection
Subcutaneous xenograft and subcutaneous tumor models

Pain

Thermal Pain
Hot Plate
Tail Flick

Mechanical Pain

von Frey Tests

Chemically-induced Inflammatory Pain
Acetic Acid-induced Writhing Test
Carrageenan-induced Inflammation
Complete Freund’s Adjuvant Challenge
Formalin Pain Assay
Mechanical Pain
Automated von Frey Tests
Manual von Frey

Neuropathic Pain Models
Chronic Constrictive Injury (CCI) Model
Neuritis (Rat)
Partial Sciatic Nerve Ligation (PNL) Model
Tibial Nerve Transection (TNT) Model

CNS and Behavior

Elevated Plus Maze for Anxiety
Feeding Behavior (Food Intake)
In Vivo Kainic Acid Challenge
Irwin Observational Battery
Long Term Monitoring for Activity
Novel Environment-induced Feeding Suppression for Anxiety
Novel Object Recognition for Learning and Memory
Open Field for Locomotion and Anxiety
Pentylenetrazole- (PTZ-) induced Seizures
Rotarod Assay for Motor Coordination
Startle Amplitude and Pre-Pulse Inhibition for Schizophrenia
Tail Suspension Test for Depression
Serum Corticosterone

Sexual Health

Female Contact Sexual Behavior
Forced Erection Test
Male Contact Sexual Behavior
Male Fertility
Serum Testosterone Levels

Clinical Chemistries

Automated Clinical Blood Chemistry Panel
Urinalysis (including urine collection and biochemical analysis)