Immunology Assays

The availability of inbred strains, having similar immune response within a strain and the wealth of experimental models that closely resemble human diseases make mice and rats a preferred system for pre-clinical research in immunology. Mice and rats are well-characterized animal species with respect to the expression of surface markers by lymphocytes and other immune competent cells. In addition, well-developed technological tools for manipulating mouse and rat genome are available.

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Our research team offers a broad variety of induced disease models and other functional in vivo and ex vivo assays to study effects of genetic manipulations and natural mutations on the immune response.

Animal Disease Models

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Antigen-induced Arthritis for Rheumatoid Arthritis
Collagen-Induced Arthritis for Rheumatoid Arthritis
KRN-serum Induced Arthritis for Rheumatoid Arthritis
Monoclonal Antibody Induced Arthritis for Rheumatoid Arthritis
Lethal Endotoxemia (Septic Shock)
Experimental Autoimmune Encephalitis for Multiple Sclerosis
Allergen-induced Pulmonary Inflammation for Asthma
Lipopolysaccharides (LPS)-induced Airway Inflammation
Acute lung injury model (LPS-induced)
DSS-induced Colitis for Ulcerative Colitis
TNBS-induced Colitis for Crohn’s Disease
Allergic Dermatitis (DNFB or Oxazolone induced)

Other in vivo Bioassays

Non-lethal Endotoxemia with LPS
Antigen-dependent In Vivo T-cell Proliferation by BrdU uptake
OVA-stimulated In Vivo Antibody Production (IgG1, IgG2a, IgG2b)
KLH-stimulated In Vivo Antibody Production (IgM, IgG)
Delayed Type Hypersensitivity by mBSA or KLH with adjuvant
Delayed Type Hypersensitivity by SRBC
Monocyte or Neutrophil Infiltration induced by Thioglycollate or Zymosan or Curdlan or Tripsin
Cutaneous Wound Healing

Ex vivo Bioassays

Serum and Tissue Cytokine Profiling in animal disease models
FACS Analysis of Lymphoid Organs and Tissue Infiltrates in Naïve and Disease Induced Animals
Hematology Five Parts Differential Cell Count
In vitro B cell proliferation Induced by LPS
In vitro T cell Proliferative Responses Induced by Anti-CD3/CD28
Cytokine Production by T cells after stimulation with anti-CD3/CD28
Cytokine Production by Monocytes after stimulation with LPS Cytotoxic T-cell Activity (CTL)
LAK- Lymphokine-Activated Killer Cell Cytotoxicity
NK- Natural Killer Cell Activity
Th17 Polarizing Conditions from CD4+ T cells (ELISA for Il-17 and IL-22)
Treg Polarization from CD4+ T cells (ELISA for IL-10)
Th1 Differentiation from CD4+ T cells (ELISA for INF-gamma)
Th2 Differentiation from CD4+ T cells (ELISA for IL-4)