Invivotek

Metabolic and Cardiovascular Assays


The Invivotek’s phenotyping program focuses on both energy homeostasis, glucohomeostasis and cardiovascular health. Energy homeostasis is measured by caloric intake, metabolic rate, body weight, body composition and physical activity. Glucohomeostasis is measured in blood glucose levels during glucose tolerance tests, insulin tolerance tests and additional chemistries. Cardiovascular health can also be assessed by blood pressure measurements and blood lipid chemistries.

Energy Homeostasis and Body Composition

All of the assays can be performed on regular and/or high fat diet
Longitudinal Body Weight Gain
Body Composition by DEXA:

  • Fat mass/ % fat/ lean mass/ % lean/ total tissue mass/ tissue area
  • Bone Mineral Density and Content/ bone area

Assessment of Regional Adipose Depots
Aipocyte tissue macrophage measurement by FACS
Food Intake
Metabolic Parameters and Activity in CCMS:

  • O2 Consumption/ CO2 Production/ Respiratory Exchange Ratio (RER)
  • Total Horizontal / Ambulatory/ Non-ambulatory/ Vertical Activity

Licking Frequency

Glucose Homeostasis

Glucose and Insulin Levels in the Oral Glucose Tolerance Test
Gastric Emptying
Insulin Tolerance Test
Tissue Glycogen Levels
Pancreatic Insulin Content
Glucagon Levels
Serum Adiponectin Levels
Specialized Blood Chemistries
Serum Corticosterone Levels
Serum Leptin Levels
Serum Ketone Levels
Serum Triglyceride Levels
Serum Free Fatty Acids
Serum Cholesterol Levels
Cholesterol Distribution: VLDL, LDL, HDL

Cardiovascular Functions

Hypertension
Non-invasive Blood Pressure & Heart Rate
Exercise capacity
High Salt or High Fat Dietary Challenge
L-NMMA Induced Hypertension

Thrombosis

Activated Partial Thromboplastin Time (aPTT)
Collagen-induced Platelet Aggregation
Prothrombin Time (PT)
Thrombin-antithrombin III complex (TAT)
Tail Bleeding Time